Whatman Filtration for HPLC Sample Preparation
Simplify sample preparation with the Whatman regenerated cellulose membrane portfolio from Cytiva
Please find in the training section, interesting papers relating to analytical HPLC from Cytiva
Accurate. Reliable. Fast
- Reduce cost with well-designed devices – the regenerated cellulose (RC) family includes products for robotic sample handing, outside-of-lab sample preparation, certified method development, and working with hard-to-filter samples.
- Increase accuracy with reliable, repeatable results – The RC family is demonstrated to have extremely low levels of chemical extractables that could interfere with analysis. Products are available to retain a variety of particle sizes.
- Save time with multifunctional products – reduce time spent deciding which membrane type to use for your sample preparation. The RC membrane is broadly compatible with common HPLC sample solvents and mobile phases.
- Improve efficiency with ready-to-use products – RC products are supplied ready for use. The hydrophilic, low-protein binding RC membrane is suitable for a wide variety of HPLC applications.
Characteristics of common membranes
Solvent compatibility | Qualities |
Membrane1 | Aqueous | Nonaqueous | Low protein binding | Low extractables |
| RC | +++ | +++ | +++ | +++ |
| CA | +++ | - | +++ | + |
| ME | +++ | + | + | - |
| CN | +++ | - | - | - |
| PVDF | +++ | +++ | +++ | + |
| PP | +++ | +++ | - | - |
| NYL | +++ | + | - | - |
| PES | +++ | + | +++ | +++ |
| PTFE | - | +++ | - | + |
| Anopore™ | +++ | + | +++ | +++ |
| PC | +++ | + | +++ | +++ |
| 1 | RC = regenerated cellulose; CA = cellulose acetate; ME = mixed ester; CN = cellulose nitrate; PVDF = polyvinylidene fluoride; PP = polypropylene; NYL = nylon; PES = polyethersulfone; PTFE = Polytetrafluoroethylene; PC = polycarbonate. |
| +++ | strong characteristic |
| + | weak characteristic |
| - | does not have characteristi |
Mobile phase filtration
Whatman RC55 and GV 050/2 vacuum filtration unit for solvent filtration
Use the same material for mobile phase filtration and sample filtration to:
- Reduce variation of analysis
- Reduce rate of column clogging
- Enhance lifetime of column
- Nylon – when mobile phase is > 20% aqueous
- Polypropylene – for non-aqueous solvents
RC Membrane
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Request NowPreventive care: Puradisc filters
Filtration of your samples is important as a preventive maintenance step for HPLC or UHPLC analysis. Keep unwanted particulate matter from entering the injector to increase column life, shorten run time, and optimize peak shape.
- Manufactured to a high standard in Cytiva’s ISO 9001:2015 certified facilities
- Available in non-RC membrane types to support a variety of other applications
Typical data
Puradic Syringe Filters
4 mm diameter* | 30 mm diameter* | |
| Housing | Polypropylene | Polycarbonate |
| Filtration area | 0.2 cm2 | 5.7 cm2 |
| Maximum pressure | 75 psi (5.2 bar) | 100 psi (6.9 bar) |
| Volume ‘hold up’ with air purge | < 10 μl | < 50 μl |
| Dimensions | 10.1 × 23.5 mm 19.5 × 7.7 mm (PVDF membrane only) | 26 × 34 mm |
| Weight (approx) | 0.55 g | 4.7 g |
| Volume throughput | up to 2 ml | up to 100 ml |
| Inlet connection | Female Luer lock | Female Luer lock |
| Outlet connection | Male Luer/tube tip | Male Luer/tube tip |
| Sterilization§ | Autoclave at 121°C | Autoclaving not recommended |
| * | Diameters available for Puradisc only. |
| § | Applies to nonsterile filters only. Do not autoclave sterile filters |
Certified quality for method development: SPARTAN filters
SPARTAN brand syringe filters are HPLC-certified for confidence and consistent results. Tested and certified for the absence of UV-absorbing substances (210 and 254 nm) with water, methanol, acetonitrile to ensure absence of interfering substances.
- Hydrophilic, low protein-binding membrane made of regenerated cellulose
- Excellent chemical resistance against the standard aqueous and organic HPLC solvents
- Tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254 nm with water, methanol, and acetonitrile
- 13 mm diameter with Mini-Tip options
- 13 mm diameter with extremely low dead volume < 10 μL
High-particulate, challenging sample filtration: Whatman GD/X filters
Filter even the most difficult samples and use less hand pressure with GD/X syringe filters.
- Exceptional loading capacity and fast flow rates – prevents back pressure and clogging of membrane
- Graduated microfiber prefilter from 1 μm to 0.7 μm
- Higher flow rates (3×) compared to unprotected membranes
- Uses glass microfiber-based prefilters
Typical data
Whatman GD/X Syringe Filters
Membrane | GD/X 13 mm | GD/X 25 mm |
| Housing | Polypropylene (pigment-free) | Polypropylene (pigment-free) |
| Filtration area | 1.3 cm2 | 4.6 cm2 |
| Maximum pressure | 100 psi (6.9 bar) | 75 psi (5.2 bar) |
| Volume “hold-up” full housing with air purge | 0.5 ml 50 μl (approx) | 1.4 ml 250 μl (approx) |
| Dimensions | 21.6 × 29.8 mm | 20.8 × 29.8 mm |
| Weight | 3 g (approx) | 3 g (approx) |
| Flow direction | Flow should enter from the inlet | Flow should enter from the inlet |
| Inlet connection | Female Luer lock | Female Luer lock |
| Outlet connection | Male Luer | Male Luer |
| Sterilization* | Autoclave at 121°C at 15 psi for 20 min | Autoclave at 121°C at 15 psi for 20 min |
| Glass microfiber prefiltration media | 100% borosilicate GMF 150: 1 μm GF/F 0.7 μm | 100% borosilicate GMF 150: 1 μm GF/F 0.7 μm |
| * | Applies to nonsterile filters only. Do not autoclave sterile GD/X filters. |
Typical data
GD/XP Syringe Filters
GD/XP 13 mm | |
| Housing | Polypropylene (pigment-free) |
| Filtration area | 4.6 cm2 |
| Maximum pressure | 75 psi (5.2 bar) |
| Volume “hold-up” full housing | 1.4 ml with air purge 250 μl (approx) |
| Dimensions | 20.8 × 29.8 mm |
| Weight | 3 g (approx) |
| Flow direction | Flow should enter from the inlet |
| Inlet connection | Female Luer lock |
| Outlet connection | Male Luer |
| Sterilization† | Autoclave at 121°C at 15 psi for 20 min |
| Prefiltration media | PP 20 μm: 5 μm |
| † | Not recommended for nylon. |
Whatman GD/X 13mm diameter
Buy NowWhatman GD/X 25mm diameter
Buy NowWhatman GD/XP 25mm diameter
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Request NowAutosampler and workflow support: Mini-UniPrep filters
Whatman Mini-UniPrep Syringeless Filters - polypropylene or glass chambers
The Mini-UniPrep syringeless filters are compatible with most autosamplers.
- Easy-to-use design supports sample preparation outside of the lab if needed
- Process samples in one-third the time of traditional syringe filtration
- Replaces syringe, syringe filter, vial, and cap in one consumable
- Polypropylene or glass chamber options to prevent interference from chemical leaching
- Amber vials available for light sensitive samples
- Multi-compressors available for ease of use
- 12 × 33 mm vial can be used to filter up to 400 μL
Typical data
Mini-UniPrep and Mini-UniPrep G2 filter vials
Mini-UniPrep | Mini-UniPrep G2 | |
| Dimensions | Equivalent in size to 12 mm × 32 mm vials | Equivalent in size to 12 × 32 mm vials |
| Materials of construction | ||
| Chamber: | Polypropylene | Borosilicate glass |
| Plunger housing: | Polypropylene | Polypropylene |
| Plunger inner storage vial | N/A | Borosilicate glass/td> |
| Filter medium: | As specified | As specified |
| Septum: | Silicone with PTFE liner | Silicone with PTFE liner |
| Cap: | Polypropylene | Polypropylene |
| Maximum operating temp | 50°C (122°F) | 50°C (122°F) |
| Max. unfiltered sample capacity | 400 μL | 500 μL |
| Max. filtered sample capacity | 350 μL | 330 μL |
| Dead volume | 50 μL | 170 μL |
| Recommended minimum filtering volume | 100 μL | 220 μL placed in the chamber to obtain 50 μL in inner storage vial |
| Nominal force needed to compress | Approx. 8.2 kg (18 lbs) | Approx. 11.3 kg (25 lbs) |
| Autosampler needle height adjustment: | 3 mm from the bottom of Mini-UniPrep | 5 mm from the bottom of Mini-UniPrep G2 |
| Autosampler compatibility | Any autosampler that accommodates standard 12 × 32 mm profile vials | Any autosampler that accommodates standard 12 × 32 mm profile vials |
Mini-UniPrep Compressors
Buy NowMini-UniPrep filter vial polypropylene housing 0.2um pore size
Buy NowMini-UniPrep filter vial polypropylene housing 0.45um pore size
Buy NowMini-UniPrep G2 filter vials with inner glass storage vial 0.2um pore size
Buy NowMini-UniPrep G2 filter vials with inner glass storage vial 0.45um pore size
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Order your free samples today so you can see for yourself how Whatman™ products can simplify your sample preparation.
There are over 100 samples available to choose from within the regenerated cellulose membrane portfolio. Please select up to a total of four samples by clicking on the products below. You can use the drop down box and search functionality to assist you. Once you have selected your products please click the next step button, where you will be asked to complete your contact details. If you've accidentally selected the wrong sample, simply click it on the right-hand side to deselect.
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