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Janelia Fluor 585, SE
Orange dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, two-photon excitation and live cell imaging
Marca: Tocris Bioscience 6418/2
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Descripción
Orange fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag™ and SNAP-tag™. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 585 nm; emission maximum = 609 nm; Quantum yield = 0.78; Max extinction coefficient = 156,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0990. Exhibits low non-specific background staining.To measure the absorbance spectrum of this dye we recommend the following solvent: trifluoroethanol plus 0.1% TFA.
We also offer Janelia Fluor™ conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.
HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Specifications
| 1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-7-(3,3-difluoro-1-azetidinyl)-9,9-dimethyl-2(9 H)-anthracenylidene]-3,3-difluoroazetidinium, inner salt | |
| 2 mg | |
| C34H27F4N3O6 | |
| 95% |
| 1811539-88-4 | |
| Amine-Reactive Janelia Fluor™ Dyes | |
| Observed MW: 649.6 |