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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Marca: Applied Biosystems™ 4387391M
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Descripción
- Recommended for RNA pathogen amplification
- Consistent amplification of RNA targets with high specificity and sensitivity
- Optimized to work with your target-specific primers and probes
- Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
Especificaciones
| Sufficient for 1000 reactions of 25 μL, contains: • 14 mL 2 x RT-PCR Buffer • 1100 μL 25 x RT-PCR Enzyme Mix • 25 mL Nuclease-free Water. |
|
| Sonda de cebado | |
| High | |
| ADN polimerasa AmpliTaq Gold | |
| Sistema 7500 | |
| AgPath-ID™, Ambion™ | |
| 1000 Reactions | |
| 1000 reacciones | |
| 25 X | |
| 14 mL |
| Works on all AB platforms, but is optimized for the 7500. | |
| Tubo | |
| 1-step RT-qPCR | |
| Estándar | |
| ROX (premezclado) | |
| One-Step RT-PCR Reagent | |
| ArrayScript™ | |
| 25X | |
| 1000 reacciones |
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Título del producto
For Research Use Only. Not for use in diagnostic procedures.
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