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Applied Biosystems™ GeneAmp™ RNA PCR Core Kit
Tienda Applied Biosystems ProductosDescripción
Includes
MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, RNase Inhibitor, dNTPs, GeneAmp 10X PCR Buffer, MgCl2 Solution, Oligo d(T)16, and Random Hexamers
The Applied Biosystems GeneAmp RNA PCR Core Kit contains all components necessary to reverse transcribe RNA to cDNA using MuLV(recombinant Moloney murineleukemia virus) reverse transcriptase and to subsequently amplify synthesized cDNA using PCR process.
- RNA reverse transcription and cDNA amplification combined in a single tube provides exceptional sensitivity
- Kit protocol enables users to perform a variety of studies including: amplification of cDNA derived from all types of RNA sequences,analysis of gene expression at the RNA level, and detection of specific RNA species present at very low copy number
- Easy to Use: Both the reverse transcription and amplification reactions take place in a single tube with buffers and reagents co-optimized for these sequential reactions
- Final product size: 5kb or less
- Sufficient for 100 reverse transcription reactions, 20μL each and 100 PCR amplifications, 100μL each
See user's manual or package insert for limited label license and trademark information.
PCR & Real-Time PCR, RT-PCR, Reverse Transcription, Two-Step RT-PCR
Información de órdenes
Condición de envío: Hielo seco
Especificaciones
Especificaciones
| Contenido y almacenamiento | MuLV Reverse Transcriptase, AmpliTaq DNA Polymerase, RNase Inhibitor, dNTPs, GeneAmp 10X PCR Buffer, MgCl2 Solution, Oligo d(T)16, and Random Hexamers. Store at -20deg.C. |
| Método de detección | Sonda de cebado |
| Cantidad | 100 reactions each (RT and PCR) |
| Para utilizar con (aplicación) | RT-PCR |
| Tipo de muestra | ARN |
| Volumen | 20μL (RT), 100μL (PCR) |
Para uso exclusivo en investigación. No apto para uso diagnóstico o terapéutico en humanos ni en animales.
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