Calpastatin Monoclonal Antibody (1F7E3D10), Invitrogen™

Descripción

MA3-944 detects calpastatin from bovine, human, pig and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin. MA3-944 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins from bovine skeletal muscle extract which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunocytochemical staining of calpastatin in porcine LLC-PK1 cells with MA3-944 results in diffuse cytoplasmic staining. The MA3-944 antigen is purified bovine skeletal muscle 80 kDa subunit of m-calpastatin. This antibody recognizes an epitope between amino acid residues 543-673 (domain IV) of human calpastatin.

Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins.