CD42b Monoclonal Antibody (HIP1), NovaFluor™ Yellow 610, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb. Applications Reported: This HIP1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIP1 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 μL (0.5 μg) per test Our internal testing shows that NovaFluor Yellow 610 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. Other strains have not been tested. See the Antibody Testing Data for an example of this strain-dependent difference. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Use 5 μL of CellBlox Blocking Buffer per stained cell sample containing 10^3 to 10^8 cells. NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow) Laser