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Thermo Scientific™ CellSensor™ CRE HEK 293T DA Assay Kit
The CellSensor™ CRE HEK 293T DA (Division Arrested) cells contains a beta-lactamase (bla) reporter gene under control of a cAMP response element (CRE) stably integrated into HEK293T cells.
Marca: Thermo Scientific™ K1457
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Descripción
The CellSensor™ CRE HEK 293T DA (Division Arrested) cells contains a beta-lactamase (bla) reporter gene under control of a cAMP response element (CRE) stably integrated into HEK293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the CRE pathway by forskolin. The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of forskolin. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can be used in other assays where a sensitive readout to intracellular changes to cAMP is needed.
Especificaciones
| Células de división detenida | |
| GPCR, vía de señalización | |
| CellSensor™ | |
| Células de división detenida | |
| Ruta de señalización |
| Cada kit contiene cantidades suficientes de células de división detenida (DA) y sustrato para ensayar en 1 placa de 384 pocillos. (Otros materiales se solicitan por separado; consulte el protocolo). Incluye: o Células CellSensor™ CRE HEK293T DA o Kit de carga LiveBLAzer™-FRET B/G, 70 µg o Solución D o Protocolo de ensayo CellSensor™ CRE HEK293T CHO-K1 o Protocolo de carga LiveBLAzer™-FRET B/G o Certificado de análisis Las células CellSensor™ CRE HEK 293T DA se envían en hielo seco y deben almacenarse en nitrógeno líquido inmediatamente después de su recepción. |
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| Células CellSensor™ CRE HEK293T DA, kit de carga LiveBLAzer™-FRET B/G, 70 μg, solución D, protocolo de ensayo CellSensor™ CRE HEK293T CHO-K1, protocolo de carga LiveBLAzer™-FRET B/G, certificado de análisis | |
| 1 placa | |
| CellSensor™ |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.