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Invitrogen™ Click-iT™ Plus EdU Alexa Fluor™ 594 Flow Cytometry Assay Kit

Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit

Marca: Invitrogen™ C10646

533.40 EUR hasta el 2025-03-16
Use el código promocional "24070" para aplicar la oferta.

Código de producto. 15574394

762.00€ / paquete de 50

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Para beneficiarse del descuento, los clientes deben comprar tres unidades del mismo producto al precio de catálogo en un solo pedido para recibir el 33.33% de descuento No hay límite para los múltiplos de 3 que los clientes pueden comprar PCODE:24111

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Descripción

The Click-iT Plus EdU Alexa Fluor 594 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods. Newly synthesized DNA is analyzed using the 532- or 561-nm laser of the flow cytometer. The Click-iT Plus formulation is compatible with standard fluorophores, including R-PE and R-PE tandems, as well as fluorescent proteins.

Multiplexable—compatible with R-PE (and tandems) and fluorescent proteins

Accurate—superior results compared to BrdU assays

Fast—results in as little as 60 minutes

View selection guide for all Click-iT EdU and Click-iT Plus EdU assays for flow cytometry.

Multiplexable
Click-iT Plus Alexa Fluor 594 EdU assays can be used in conjunction with R-PE and R-PE tandems, as well as fluorescent proteins such as GFP and mCherry. The Alexa Fluor 594 labeling reagent is excited readily at either 532 or 561 nm and emits at 615 nm.

An Advanced Method Giving You Results Superior to BrdU
The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Originally, this was performed through incorporation of radioactive nucleosides. This method was replaced by antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). The Click-iT Plus EdU Flow Cytometry assay is a novel alternative to the BrdU assay. EdU (5-ethynyl-2´-deoxyuridine) is a thymidine analog that is incorporated into DNA during active DNA synthesis. Detection is based on click chemistry, which is a copper-catalyzed covalent reaction between an azide and an alkyne. In this application, the alkyne is found in the ethynyl moiety of EdU, while the azide is coupled to the Alexa Fluor dye. Standard flow cytometry methods are used for determining the percentage of S-phase cells in the population.

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies
The small size of the dye azide allows for efficient detection of the incorporated EdU using mild conditions, while standard aldehyde-based fixation and detergent permeabilization are sufficient for the Click-iT Plus detection reagent to gain access to the DNA. This is in contrast to BrdU assays that require DNA denaturation (using HCl, heat, or digestion with DNase) to expose the BrdU so that it can be detected with an anti-BrdU antibody. Sample processing for the BrdU assay can result in signal alteration of the cell cycle distribution, as well as destruction of antigen recognition sites when using the HCl method. In contrast, the easy-to-use Click-iT Plus EdU assay is compatible with cell cycle dyes. The Click-iT Plus EdU assay can also be multiplexed with antibodies against surface and intracellular markers, as well as conjugates labeled with standard fluorophores including R-PE, R-PE tandems, and fluorescent proteins (GFP and mCherry).

Quick and Simple Protocol
The Click-iT Plus EdU protocol is based on the aldehyde fixation and detergent permeabilization steps for immunohistochemical antibody labeling. However, EdU is compatible with other fixation/permeabilization agents including saponin and methanol. In just five steps you’ll be ready to analyze your cell proliferation data:

1. Treat cells with EdU.
2. Fix and permeabilize cells.
3. Detect S-phase cells with Click-iT Plus detection cocktail for 30 min.
4. Wash once.
5. Analyze.

Results can be seen in as little as 60 minutes in some circumstances, but we recommend 90 minutes for all applications.

Especificaciones

Cantidad	50 assays
Excitación/emisión	532 or 561⁄615
Línea de productos	Alexa Fluor™, Click-iT™

Contenido y almacenamiento	Contains EdU (5-ethynyl-2' -deoxyuridine), AlexaFluor 594 picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT fixative, Click-iT saponin-based permeabilization and wash buffer, Copper Protectant, and Click-iT EdU buffer additive. Store at 2–8°C. Dessicate and protect from light.
Condiciones de envío	Room Temperature

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Los clientes que vieron este artículo también vieron This information does not imply a recommendation or representation of any kind and any action taken upon the information provided is strictly at your own risk. The products shown have not been qualified by Fisher Scientific for any particular purpose, application or use and it is the responsibility of the purchaser to ensure that the performance of the product is suitable for the purchasers specific use or intended application.

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Documentos

Product Information

Manuals

User Guide: Click-iT Plus EdU Flow Cytometry Assay Kits Versión: E.0 Tamaño : 501.2 kB

Certificados

Se requiere un número de lote para mostrar resultados para certificados. Para encontrar su número de lote en pedidos anteriores, utilice el área de estado de pedidos.

Número de lote

5 Resultados encontrados

Número de lote	Tipo de certificado	Fecha	Catalog Number
Número de lote2961096	Tipo de certificadoCertificado de análisis	Fecha27/08/2024	Catalog NumberC10646
Número de lote2845255	Tipo de certificadoCertificado de análisis	Fecha22/02/2024	Catalog NumberC10646
Número de lote2696835	Tipo de certificadoCertificado de análisis	Fecha24/07/2023	Catalog NumberC10646
Número de lote2581642	Tipo de certificadoCertificado de análisis	Fecha20/03/2023	Catalog NumberC10646
Número de lote2502980	Tipo de certificadoCertificado de análisis	Fecha29/06/2022	Catalog NumberC10646

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Invitrogen™ Click-iT™ Plus EdU Alexa Fluor™ 594 Flow Cytometry Assay Kit

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Product Information

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Certificados

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