Invitrogen™ Kit de ELISA competitivo de cortisol humano

Descripción

The Human Cortisol ELISA quantitates Cortisol in human serum,plasma,urine,saliva,dried fecal extracts or or cell culture medium.

Principle of the method

The Cortisol Competitive ELISA research-use-only kit is designed to quantitatively measure cortisol present in dried fecal extracts,saliva,urine,serum,plasma,and tissue culture media samples. Total cortisol is measured in extracted samples and in serum and plasma,and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

El cortisol es el glucocorticoide más potente producido por la glándula suprarrenal humana. Se sintetiza a partir del colesterol y su producción es estimulada por la hormona adrenocorticotrópica hipofisaria (ACTH), que está regulada por el factor liberador de corticotropina (CRF). Las secreciones de ACTH y CRF se inhiben por los altos niveles de cortisol en un bucle de retroalimentación negativa. En el plasma, la mayoría del cortisol se une con alta afinidad a la globulina enlazante de corticosteroides (CBG o transcotina). El cortisol actúa a través de receptores intracelulares específicos y afecta a numerosos sistemas fisiológicos, incluyendo la función inmune, la regulación de la glucosa, el tono vascular y el metabolismo óseo.