Thermo Scientific™ Columnas de RP DNAPac™

Descripción

Consiga separaciones de oligonucleótidos de fase reversa superiores con las columnas para HPLC Thermo Scientific™ DNAPac™ RP. La química exclusiva está diseñada para el análisis de oligonucleótidos y fragmentos de ADN/ARN de doble cadena (ds) mediante LC-UV o LC-MS. La química de las columnas proporciona un rendimiento excelente bajo una amplia gama de composiciones de pH, temperatura y fase móvil. Además, el ancho tamaño de poro de la resina proporciona una excelente separación de ácidos nucleicos de doble cadena grandes de hasta 10 k de pares de bases.

DNAPac RP reversed phase (RP) column is based on spherical wide-pore 4 μm polymer resin specifically designed for separation of oligonucleotides and DNA/RNA fragments. The stationary phase is highly stable to extreme pH (0-14), and temperature (5-110°C) and fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA). The wide pore size of polymer resin enables efficient separation of small to large nucleic acids with low carry-over. The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments:

• Designed for ion-pair reversed-phase (IP-RP) LC and LC/MS separations of both oligonucleotides and double-stranded nucleic acids
• High resolution and high throughput
• Excellent MS compatibility
• Wide operating pH range (0-14) and high temperature stability (up to 110°C)
• Biocompatible hardware to eliminate analyte absorption