Thermo Scientific™ Kits de preparación de muestras para MS EasyPep™

Descripción

Los kits de preparación de muestras para MS EasyPep Mini permiten el procesamiento eficaz y reproducible de células y tejidos cultivados de mamíferos para el análisis proteómico de MS. El kit contiene tampones preformulados, mezcla de enzimas de grado MS, columnas de limpieza de péptidos y un protocolo optimizado para generar muestras de péptidos compatibles con MS en menos de tres horas. Los kits están optimizados para procesar muestras de proteínas de 10 a 100 µg con un alto rendimiento de péptidos preparados para MS.

• Complete—includes pre-formulated reagents for lysis through digestion, peptide clean-up columns, and an optimized protocol for up to 20 samples
• Optimized—streamlined protocol and reagents minimize the number of steps and time time needed to process samples
• Flexible—reagents and protocol have been verified using cells, plasma, and tissue samples for 10 to 100 μg samples
• Time-saving—sample processing time reduced from more than one day to less than three hours
• Compatible—final preparation is ready for direct MS analysis and other downstream applications, including TMT labeling

The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in sample variability in both quality and quantity. This kit has been designed to improve reproducibility while saving hands-on and processing time. The number of steps and processing time have been reduced through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes Peptide Clean-up columns and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or are ready for MS analysis.