PeproTech Eotaxin 2 Polyclonal Antibody, PeproTech®, Invitrogen™

Description

AA Sequence of recombinant protein: VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKGGVIFT TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human Eotaxin-2 (CCL24). Anti-Human Eotaxin-2 (CCL24)-specific antibody was purified by affinity chromatography employing an immobilized Human Eotaxin-2 (CCL24) matrix. Sandwich ELISA: To detect Human Eotaxin-2 (CCL24) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Eotaxin-2 (CCL24) (500-P103GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Eotaxin-2 (CCL24). Western Blot: To detect Human Eotaxin-2 (CCL24) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Eotaxin-2 (CCL24) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.