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R&D Systems™ Recombinant F. meningosepticum PNGase F Protein, CF
Effective removal of N-linked oligosaccharides from glycoproteins. Glycerol-free, suitable for downstream MS and HPLC. Get 50% off! Use Promo Code: PNGaseF50 Terms and Conditions
Marca: R&D Systems™ 9109-GH-020
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Descripción
Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.Especificaciones
| P21163 | |
| M.W. (Observed): 34 kDa, reducing conditions; M.W. (theoretical): 36 kDa | |
| E. coli-derived f. meningosepticum PNGase F protein Ala41-Asn354 with N-terminal Met and 6-His tag | |
| <1.0 EU / 1 µg of the protein by the LAL method. | |
| Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions. | |
| Yes |
| Supplied as a 0.2 µm filtered solution in Tris and NaCl. | |
| 20 µg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
| PNGase F | |
| Unconjugated |
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