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Thermo Scientific™ GeneBLAzer™ OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cells
The GeneBLAzer™ OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cell Line contains the human opioid/opiate receptor-like-1 receptor (OPRL1) stably integrated into the GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F Cell Line.
Marca: Thermo Scientific™ K1714
Descripción
The GeneBLAzer™ OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F Cell Line contains the human opioid/opiate receptor-like-1 receptor (OPRL1) stably integrated into the GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F Cell Line. GeneBLAzer™ Gqi5-NFAT-bla FreeStyle™ 293F cells (Cat. No. K1539) contain the chimeric Gqi5 G protein and the beta-lactamase reporter gene under control of the nuclear factor of activated T cells (NFAT) response element.
OPRL1-Gqi5-NFAT-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and functionally validated for Z-factor and EC50 concentrations of orphanin FQ/nociceptin. These data are found in the Validation & Assay Performance Summary.
Orphanin FQ/nociceptin and OPRL1 have been implicated in various physiological functions. The most defined role is their involvement in the mediation of pain in the central nervous system and their possible roles in opiate tolerance, opiate dependence and withdrawal, and the ability to cope with stress and anxiety. Through the use of antagonists, antisense oligos, and transgenic knockout mutants, OPRL1 has been implicated in additional systems including learning, memory, and reproduction.
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Especificaciones
NM_000913 | |
Células divisoras | |
Fluorescente | |
OPRL1 | |
Origen del clon unicelular | |
2 x 106 células | |
OPRL1 | |
Células FreeStyle 293F | |
GPCR |
Gen informador de beta lactamasa a base de células | |
Contenido: Células K1714 GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F (El kit de carga LiveBLAzer™-FRET B⁄G, la solución D y otros materiales se solicitan por separado; consulte el protocolo). Incluye: • Células GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F • Protocolo de ensayo GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F • Certificado de análisis Almacenamiento: Las células GeneBLAzer™ OPRL1-Gqi5-NFAT-bla Freestyle 293F se envían en hielo seco y deben almacenarse en nitrógeno líquido inmediatamente después de su recepción. |
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FRET | |
Células OPRL1-Gqi5-NFAT-bla Freestyle 293F GeneBLAzer™, ensayo OPRL1-Gqi5-NFAT-bla Freestyle 293F GeneBLAzer™ | |
GeneBLAzer™ | |
BLA (betalactamasa) | |
Célula 293F | |
GeneBLAzer™ |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.