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Thermo Scientific™ GeneBLAzer™ RARα-UAS-bla HEK 293T Cells
RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK293T cell line.
Marca: Thermo Scientific™ K1685
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Descripción
RAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor alpha fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK293T cell line. GeneBLAzer™UAS-bla HEK 293T cells (Cat. No. K1104) stably express a beta-lactamase reporter gene under the transcriptional control of a 7X Upstream Activator Sequence (UAS). Transcription from the 7X UAS is activated by the binding of the GAL4 transcription factor DNA-binding-domain (DBD). The GAL4-DBD is expressed as a fusion protein with the ligand binding domain (LBD) of RAR alpha. When an agonist binds to the LBD of the GAL4(DBD)-RAR alpha(LBD) fusion protein it translocates to the nucleus where it binds to the 7X UAS inducing transcription of betalactamase. RAR alpha-UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including DMSO concentration, cell number, stimulation time, substrate loading time and have been validated for Z' and EC50 concentrations of all-trans retinoic acid. Additional testing data using alternate stimuli are also available.
Especificaciones
| Tetrac, T4 | |
| Gen informador de beta lactamasa a base de células | |
| Envío: Hielo seco Almacenamiento: -80 °C/Nitrógeno líquido Contiene: Células HEK 293T TR alfa-UAS-bla (K1260) | |
| Receptores nucleares | |
| Células TR alpha-UAS-bla HEK 293T (K1260) | |
| Hormona T3 | |
| 2 x 106 células | |
| BLA (beta-lactamasa) | |
| Células en proceso de división |
| 1-850 | |
| Células divisoras | |
| Fluorescente | |
| Receptores nucleares | |
| 2 x 106 Cells | |
| GeneBLAzer™ | |
| Célula HEK 293T | |
| RARA, RAR alfa, NR1B1 |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.