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Applied Biosystems™ HIV-1 Genotyping Kit with Integrase

The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase is designed to genotype the protease, reverse transcriptase (RT), and integrase (IN) regions of the HIV-1 pol gene. The genotyping result encompasses codons 6-99 in the protease region and codons 1-251 in the RT region of the gene.

Marca:  Applied Biosystems™ A55120

6105.41 EUR hasta el 2024-12-31
Use el código promocional "21975" para aplicar la oferta.


Código de producto. 17767517

  • 6450.00€ / paquete de 48

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Descripción

Descripción

  • Designed to genotype the protease, reverse transcriptase (RT) and integrase (IN) regions of the HIV-1 pol gene
  • The genotyping result encompasses codons 6-99 in the protease region and codons 1-251 in the RT region of the gene
  • HIV-1 genomic mutations in these regions confer resistance to anti-retroviral drugs that act through the inhibition of the protease and reverse transcriptase
  • The kit enables reliable genotyping of the genetically diverse HIV-1 virus to investigate Drug Resistance Mutations (DRMs) to protease inhibitors (PI) , nucleoside reverse-transcriptase inhibitors (NRTI), non-nucleoside reverse-transcriptase inhibitors (NNRTI), and integrase inhibitors (INSTI) from HIV-1 RNA extracted from EDTA plasma and dried blood spot samples
  • This product employs assays for HIV-1 genotyping licensed from the U.S. Centers for Disease Control and Prevention
  • Kit contains ready-to-use master mix, enzyme, and positive and negative controls to cover the three phases of the workflow: RT-PCR, nested PCR, and bidirectional cycle sequencing steps to process 48 test samples
  • Starting with viral RNA extracted from an EDTA plasma or dried blood spot sample, RT-PCR is conducted to generate PR/RT and IN PCR products as DNA template
  • After confirming the band size of the PCR amplicons, the Cycle Sequencing module is used to sequence the DNA templates utilizing Sanger sequencing technology
  • The sequencing reactions are first purified, then sequenced using POP-7 on a designated capillary electrophoresis system
  • The sequencing traces are reviewed for quality, after which the user exports the AB1 file for use in downstream analysis and reporting software a mutation profile through a curated database

Includes

  • RNA Positive Control, 2 x 100 μL
  • HIV RNA Negative Control, 2 x 100 μL
  • RT-PCR Master Mix, PR/RT, 2 x 1.055 mL
  • RT-PCR Master Mix, IN, 2 x 1.055 mL
  • Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL
  • Nested-PCR Master Mix, IN, 2 x 1,285 μL
  • SuperScript™ III One-Step RT-PCR with Platinum™ Taq High Fidelity Enzyme, 2 x 54 μL
  • AmpliTaq Gold™ LD DNA Polymerase, 1 x 54 μL
  • Sequencing vector, 2 x 80 μL
  • HIV Sequencing Mix R12, 2 x 435 μL
  • HIV Sequencing Mix R11, 2 x 435 μL
  • HIV Sequencing Mix F12, 2 x 435 μL
  • HIV Sequencing Mix F11, 2 x 435 μL
  • HIV Sequencing Mix R3, 2 x 435 μL
  • HIV Sequencing Mix R2, 2 x 435 μL
  • HIV Sequencing Mix R1, 2 x 435 μL
  • HIV Sequencing Mix F3, 2 x 435 μL
  • HIV Sequencing Mix F2, 2 x 435 μL
  • HIV Sequencing Mix F1, 2 x 435 μL

Store all components at -15°C to -25°C

TRUSTED_SUSTAINABILITY
Especificaciones

Especificaciones

• RNA Positive Control, 2 x 100 μL
• HIV RNA Negative Control, 2 x 100 μL
• RT-PCR Master Mix, PR/RT, 2 x 1.055 mL
• RT-PCR Master Mix, IN, 2 x 1.055 mL
• Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL
• Nested-PCR Master Mix, IN, 2 x 1.285 mL
• SuperScript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme, 2 x 54 μL
• AmpliTaq Gold LD DNA Polymerase, 1 x 54 μL
• Sequencing vector, 2 x 80 μL
• HIV Sequencing Mix R12, 2 x 435 μL
• HIV Sequencing Mix R11, 2 x 435 μL
• HIV Sequencing Mix F12, 2 x 435 μL
• HIV Sequencing Mix F11, 2 x 435 μL
• HIV Sequencing Mix R3, 2 x 435 μL
• HIV Sequencing Mix R2, 2 x 435 μL
• HIV Sequencing Mix R1, 2 x 435 μL
• HIV Sequencing Mix F3, 2 x 435 μL
• HIV Sequencing Mix F2, 2 x 435 μL
• HIV Sequencing Mix F1, 2 x 435 μL
Magnetic Bead
Secuenciación de ADN y ARN
Genotyping Kit
Dry Ice
DNA Staining, PCR amplification
Kit
RNA from EDTA Plasma and DBS
Veriti™ 96‐well Thermal Cycler with 0.2‐mL sample wells, GeneAmp™ PCR System 9700 96-well, ProFlex™ 96‐well PCR System with 0.2 mL sample wells, 3130 series Sequencers, 3500/3500xL Genetic Analyzer series, SeqStudio Flex Genetic Analyzers (8 or 24 caps, up to 4 plates)
48 tests
HIV-1 subtypes A, B, C, D, F, G, CRF01_AE, CRF02_AG, CRF 06_cpx.
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