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Applied Biosystems™ HIV-1 Genotyping Kit with Integrase
The Applied Biosystems™ HIV-1 Genotyping Kit with Integrase is designed to genotype the protease, reverse transcriptase (RT), and integrase (IN) regions of the HIV-1 pol gene. The genotyping result encompasses codons 6-99 in the protease region and codons 1-251 in the RT region of the gene.
Marca: Applied Biosystems™ A55120
6105.41 EUR hasta el 2024-12-31
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Descripción
- Designed to genotype the protease, reverse transcriptase (RT) and integrase (IN) regions of the HIV-1 pol gene
- The genotyping result encompasses codons 6-99 in the protease region and codons 1-251 in the RT region of the gene
- HIV-1 genomic mutations in these regions confer resistance to anti-retroviral drugs that act through the inhibition of the protease and reverse transcriptase
- The kit enables reliable genotyping of the genetically diverse HIV-1 virus to investigate Drug Resistance Mutations (DRMs) to protease inhibitors (PI) , nucleoside reverse-transcriptase inhibitors (NRTI), non-nucleoside reverse-transcriptase inhibitors (NNRTI), and integrase inhibitors (INSTI) from HIV-1 RNA extracted from EDTA plasma and dried blood spot samples
- This product employs assays for HIV-1 genotyping licensed from the U.S. Centers for Disease Control and Prevention
- Kit contains ready-to-use master mix, enzyme, and positive and negative controls to cover the three phases of the workflow: RT-PCR, nested PCR, and bidirectional cycle sequencing steps to process 48 test samples
- Starting with viral RNA extracted from an EDTA plasma or dried blood spot sample, RT-PCR is conducted to generate PR/RT and IN PCR products as DNA template
- After confirming the band size of the PCR amplicons, the Cycle Sequencing module is used to sequence the DNA templates utilizing Sanger sequencing technology
- The sequencing reactions are first purified, then sequenced using POP-7 on a designated capillary electrophoresis system
- The sequencing traces are reviewed for quality, after which the user exports the AB1 file for use in downstream analysis and reporting software a mutation profile through a curated database
Includes
- RNA Positive Control, 2 x 100 μL
- HIV RNA Negative Control, 2 x 100 μL
- RT-PCR Master Mix, PR/RT, 2 x 1.055 mL
- RT-PCR Master Mix, IN, 2 x 1.055 mL
- Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL
- Nested-PCR Master Mix, IN, 2 x 1,285 μL
- SuperScript™ III One-Step RT-PCR with Platinum™ Taq High Fidelity Enzyme, 2 x 54 μL
- AmpliTaq Gold™ LD DNA Polymerase, 1 x 54 μL
- Sequencing vector, 2 x 80 μL
- HIV Sequencing Mix R12, 2 x 435 μL
- HIV Sequencing Mix R11, 2 x 435 μL
- HIV Sequencing Mix F12, 2 x 435 μL
- HIV Sequencing Mix F11, 2 x 435 μL
- HIV Sequencing Mix R3, 2 x 435 μL
- HIV Sequencing Mix R2, 2 x 435 μL
- HIV Sequencing Mix R1, 2 x 435 μL
- HIV Sequencing Mix F3, 2 x 435 μL
- HIV Sequencing Mix F2, 2 x 435 μL
- HIV Sequencing Mix F1, 2 x 435 μL
Store all components at -15°C to -25°C
Especificaciones
• RNA Positive Control, 2 x 100 μL • HIV RNA Negative Control, 2 x 100 μL • RT-PCR Master Mix, PR/RT, 2 x 1.055 mL • RT-PCR Master Mix, IN, 2 x 1.055 mL • Nested-PCR Master Mix, PR/RT, 2 x 1.285 mL • Nested-PCR Master Mix, IN, 2 x 1.285 mL • SuperScript III One-Step RT-PCR with Platinum Taq High Fidelity Enzyme, 2 x 54 μL • AmpliTaq Gold LD DNA Polymerase, 1 x 54 μL • Sequencing vector, 2 x 80 μL • HIV Sequencing Mix R12, 2 x 435 μL • HIV Sequencing Mix R11, 2 x 435 μL • HIV Sequencing Mix F12, 2 x 435 μL • HIV Sequencing Mix F11, 2 x 435 μL • HIV Sequencing Mix R3, 2 x 435 μL • HIV Sequencing Mix R2, 2 x 435 μL • HIV Sequencing Mix R1, 2 x 435 μL • HIV Sequencing Mix F3, 2 x 435 μL • HIV Sequencing Mix F2, 2 x 435 μL • HIV Sequencing Mix F1, 2 x 435 μL |
|
Magnetic Bead | |
Secuenciación de ADN y ARN | |
Genotyping Kit | |
Dry Ice | |
DNA Staining, PCR amplification |
Kit | |
RNA from EDTA Plasma and DBS | |
Veriti™ 96‐well Thermal Cycler with 0.2‐mL sample wells, GeneAmp™ PCR System 9700 96-well, ProFlex™ 96‐well PCR System with 0.2 mL sample wells, 3130 series Sequencers, 3500/3500xL Genetic Analyzer series, SeqStudio Flex Genetic Analyzers (8 or 24 caps, up to 4 plates) | |
48 tests | |
HIV-1 subtypes A, B, C, D, F, G, CRF01_AE, CRF02_AG, CRF 06_cpx. |