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Invitrogen™ Human IL-1 alpha ELISA Kits
Measure levels of human interleukin-1 alpha (IL-1a) in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.
Marca: Invitrogen™ EH2IL1A
Descripción
Assay IL-1a (human interleukin-1 alpha) levels in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples with our Invitrgen™ Human IL-1 alpha ELISA Kits.
These human IL-1 alpha ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-1 alpha (IL-1α) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.Target: Natural and recombinant human interleukin-1 alpha (IL-1α)
Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: Absorbance at 450nm minus absorbance at 550nm
Assay Range: 10.2 to 400pg/mL
Sensitivity: < 2pg/mL
Precision: Intra-assay CV ≤ 10%; inter-assay CV ≤ 10%
Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 3 hours
Sample Size: 50μL
Specificity: No significant cross-reaction with human IFNα, IFNγ, IL-1β, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, or mouse IL-1α. Human IL-1ra does not interfere with this ELISA
Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Especificaciones
10.2 to 400pg/mL | |
Human IL-1 alpha ELISA Kit | |
Cell Culture Supernatant, Plasma (EDTA, Citrate, Heparin), Serum, Urine | |
96 microplate wells | |
Absorbancia a 450 nm menos absorbancia a 550 nm | |
1 Plate Kit | |
Natural and recombinant human interleukin-1 alpha (IL-1a) |
<2pg/mL | |
Colorimetric ELISA (TMB substrate) with Plates | |
No significant cross-reaction with human IFNa, IFNg, IL-1ß, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, or mouse IL-1a. Human IL-1ra does not interfere with this ELISA | |
Stop Solution, 13mL, Streptavidin-HRP Concentrate, 75µL, Standard Diluent, 12mL, contains 0.01% thimerosal, Biotinylated Antibody Reagent, 8mL, TMB Substrate Solution, 13mL, Anti-Human IL-1 alpha Precoated 96-well Strip Plate, 1 ea, HRP Dilution Buffer, 14mL, Lyophilized Recombinant Human IL-1 alpha Standard, 2 vials, Adhesive Plate Covers, 6 ea, 30X Wash Buffer, 50mL | |
CV intraensayo de ≤ 10 %; CV interensayo de ≤ 10 % | |
50 μl | |
3 hr. |