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Invitrogen™ Kit de ELISA para IgG1 humana
Descripción
The Human Immunoglobulin G1 (Hu IgG1) ELISA quantitates Hu IgG1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IgG1. Principle of the method The Human IgG1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Especificaciones
Especificaciones
| Intervalo del ensayo | De 0,05 ng/ml a 40 ng/ml |
| Sensibilidad del ensayo | 50 pg/ml |
| Intervalo de calibración | De 0,05 ng/ml a 40 ng/ml |
| ensayo | ELISA sandwich de fase sólida |
| Conjugado | Biotina |
| Tipo de producto | Kit de ELISA |
| Familia de proteínas | Otras proteínas |
| Tipo de muestra | Plasma, Sobrenadante, Suero |
| Especificidad | Detecte IgG1 humana. No se ha encontrado reactividad cruzada con IgG2, IgG3, IgG4, IgM, IgA, IgD y IgE |
| Suficiente para | Ensayos de 5 x 96 |
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