DiscoverX InCELL Hunter™ eXpress RIPK2 Kinase Binding Assay

The InCELL Hunter™ eXpress RIPK2 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of RIPK2 via detection of RIPK2 levels. This product uses EFC technology. The cell line included overexpresses RIPK2 target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.