Invitrogen™ IgE Mouse ELISA Kit

Descripción

The Mouse Immunoglobulin E (Ms IgE) ELISA quantitates Ms IgE in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IgE. Principle of the method The Mouse IgE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La IgE es una de las cinco clases de inmunoglobulinas que se encuentran en los mamíferos. Los monómeros consisten en dos cadenas pesadas (epsilon) con 4 dominios constantes similares a Ig, y dos cadenas ligeras. La IgE desempeña un papel esencial en la hipersensibilidad de tipo I (respuestas alérgicas como fiebre del heno, asma, urticaria, choque anafiláctico) al unirse a los receptores Fc en basófilos y mastocitos. El entrecruzamiento de IgE unida a receptores Fc induce la desgranulación de mastocitos y basófilos que promueven manifestaciones alérgicas. La IgE también es funcionalmente importante para la inmunidad contra los parásitos.