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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit
Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.
Especificaciones
Color | Indicador de pH: Amarillo = condiciones correctas Verde = pH ligeramente alto (ajuste recomendado) Azul = pH demasiado alto (ajuste necesario) |
---|---|
Formato | Minicolumna de centrifugación |
Isolation Technology | Silica Membrane Technology |
Eficacia | Recuperación típica: 70–95 % |
Cantidad | 1 Kit |
Descripción
NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.
- Two applications in one kit—One buffer to process both sample types, with only one loading step
- High recovery for fragments down to 50 bp—Average recovery from 60–90%
- Minimized elution volume of 15μL—Highly concentrated DNA
- Binding buffer with pH indicator—Ensures optimal binding conditions
- Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
- Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
- Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
- Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)
Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer
Especificaciones
Indicador de pH: Amarillo = condiciones correctas Verde = pH ligeramente alto (ajuste recomendado) Azul = pH demasiado alto (ajuste necesario) |
|
Silica Membrane Technology | |
1 Kit | |
<400 μl (mezcla de reacción de PCR) <400 mg (gel de agarosa TAE/TBE) |
|
10 min/6 prep. |
Minicolumna de centrifugación | |
Recuperación típica: 70–95 % | |
A260/A280: 1,8–1,9 | |
50 bp to ∼20 kbp | |
Hasta ∼15 μg de ADN |
Seguridad y manipulación
- Atención
Clasificación
- Toxicidad aguda Categoría 4
- PELIGRO ACUÁTICO A LARGO PLAZO Categoría 3
Indicaciones de peligro
- H302-Nocivo en caso de ingestión.
- H412-Nocivo para los organismos acuáticos, con efectos nocivos duraderos.
Otros peligros
- MIXTURE LIST-Contém: guanidinium thiocyanate
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