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Thermo Scientific™ Phusion™ HF and GC Buffer Packs
5X Phusion Green buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.
Marca: Thermo Scientific™ F518L
21.63 EUR hasta el 2024-12-20
Use el código promocional "21651" para aplicar la oferta.
Descripción
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.
Tampón de HF para ADN polimerasas Phusion adecuado para todos los moldes estándarIncluye:
Tampón de HF 5x Phusion, solución de MgCl2 de 50 mm, DMSO al 100 %.
Especificaciones
El paquete de tampones HF Phusion (F-518L) incluye: • Tampón HF 5X Phusion (el tampón 1X proporciona 1,5 mM de MgCl2) • 50 mM de MgCl2 • DMSO Almacenar a -20 °C. |
|
ADN polimerasa de alta fidelidad Phusion | |
Wet or Dry Ice | |
ADN | |
Colorless |
Paquete de tampones Phusion™ HF | |
HF Buffer Pack | |
PCR estándar, High-fidelity PCR | |
4 x 1,5 mL |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.