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Thermo Scientific™ Kit de ensayo de citotoxicidad de LDH Pierce™
Tienda Thermo Scientific ProductosDescripción
Quantitatively measure lactose dehydrogenase (LDH) released into the media from damaged cells as a biomarker for cellular cytotoxicity and cytolysis using a colorimetric assay with Thermo Scientific™ Pierce LDH Cytotoxicity Assay Kit.
Additional Information and Online Ordering
Highlights:
Convenient – add–mix–read assay format for adherent and suspension cells
Colorimetric – quantitatively measures LDH release by formation of colored product
Robust – uses stable LDH enzyme activity as a cytotoxic marker
Flexible – ideal for high–throughput screening
Non–radioactive – safe alternative to 51Cr–release cytotoxicity assays
Includes:
Kit contains substrate mix, assay buffer, 10X lysis buffer, stop solution, and LDH positive control
Requires:
Microplate reader capable of reading absorbance at 490nm and 680nm, flat-bottom clear 96–well plate compatible with spectrophotometer, multichannel pipette
Recommended for:
- Measure in vitro cytotoxicity mediated by chemicals, immune cells, or siRNA or microRNA
- Detect cytolysis in bioreactor or 3D tissue engineering applications
Especificaciones
Especificaciones
| Descripción | Kit de ensayo de citotoxicidad de LDH Pierce |
| Cantidad | Kit de 200 reacciones |
| Contenido del kit | Control positivo de LDH, 6 µl, Stop Solution, 12mL, LDH Positive Control, 6µL, Assay Buffer, 0.6mL, 10X Lysis Buffer, 2.5mL |
| Suficiente para | 200 reacciones en placas de 96 pocillos o 800 reacciones en placas de 384 pocillos |
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