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R&D Systems™ Recombinant SARSCoV-2 B.1.1.529 RBD Alexa Fluor™ 488 Protein
Description
Measured by flow cytometry for its ability to bind HEK293 human embryonic kidney cells transfected with human ACE-2 at 0.250-1.00 μg/mL (100 μL/well). Please Note: Optimal dilutions should be determined by each laboratory for each application.
Specifications
Specifications
| Número de acceso | YP_009724390.1 |
| Para utilizar con (aplicación) | Bioactivity |
| Formulación | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Peso molecular | M.W. (Observed): 33-39 kDa, under reducing conditions., M.W. (Theoretical): 26 kDa |
| Cantidad | 20 μg |
| Fuente | Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike RBD protein Arg319-Phe541 (Gly339Asp, Ser371Leu, Ser373Pro, Ser375Phe, Lys417Asn, Asn440Lys, Gly446Ser, Ser477Asn, Thr478Lys, Glu484Ala, Gln493Arg, Gly496Ser, Gln498Arg, Asn501Tyr, Tyr505His), with a C-terminal 6-His tag |
| Requisitos de almacenamiento | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening. |
| Concentración de endotoxinas | <1.0 EU per 1 μg of the protein by the LAL method. |
| Conjugado | Alexa Fluor 488 |
| Grado de pureza o calidad | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
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