Novus Biologicals™ Red Blood Cell (RBC) Lysis Buffer

Descripción

The Red Blood Cell (RBC) Lysis Buffer is for optimal lysis of erythrocytes in single-cell suspensions of mouse hematopoietic tissues such as spleen and human peripheral blood. This buffer contains ammonium chloride, which lyses red cells with minimal effect on lymphocytes. Nucleated red blood cells are not effectively lysed with ammonium chloride. RBC lysis is not necessary when working with mouse thymus and lymph node. Buffer: Dilute 10X RBC Lysis Buffer to 1X in deionized water before use (1mL of 10X RBC lysis buffer with 9mL of deionized water). Bring to room temperature prior to use. Reconstitute: Solubilize the peptides prior to use by making 5mM PBS stock solutions