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STEMCELL Technologies StemSpan™ T Cell Generation Kit
For expansion and differentiation of human CD34+ hematopoietic progenitor cells to T cells
Marca: STEMCELL Technologies 09940
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Descripción
- Use this two-step protocol to reproducibly expand thousands of CD4+CD8+ double-positive (DP) T cells in cultures initiated with CD34+ human cord blood (CB) cells
- In step one, CD34+ CB cells are cultured for 14 days in medium containing expansion supplement to promote their proliferation and differentiation into CD7+CD5+ pro-T cells
- In step two, pro-T cells generated during those first 14 days are cultured for another 4 weeks in medium containing maturation supplement to promote their maturation into DP cells
- This product is serum- and feeder-free, which helps eliminate variation introduced by serum and stromal cell lines
- An optional protocol is available to further mature DP cells to CD8 SP T cells
- This extended protocol uses complete T cell progenitor maturation medium made with reagents included in the StemSpan T Cell Generation Kit and which must be combined with ImmunoCult CD3/CD28/CD2 T Cell Activator (Catalog #10970 or #10971) and IL-15 (Catalog #78031)
Especificaciones
| Hematopoietic Stem and Progenitor Cells, T Cells | |
| Cell Culture; Differentiation; Expansion | |
| 1 Kit |
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Título del producto
STEMCELL Technologies StemSpan™ T Cell Generation Kit
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