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Thermo Scientific™ Tango™ SSTR2-bla U2OS Cells
The Tango™ SSTR2-bla U2OS cells contain the human Somatostatin Receptor 2 (SSTR2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Marca: Thermo Scientific™ K1582
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Descripción
The Tango™ SSTR2-bla U2OS cells contain the human Somatostatin Receptor 2 (SSTR2) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.
The Tango™ SSTR2-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of Somatostatin-14. In addition, Tango™ SSTR2-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Especificaciones
| NM_001050.2 | |
| Células divisoras | |
| Cola de AVPR2 GPCR | |
| GPCR | |
| Células SSTR2-bla U2OS, protocolo de ensayo SSTR2-bla U2OS, certificado de análisis | |
| Tango™ | |
| BLA (betalactamasa) | |
| SSTR2 | |
| U2OS | |
| GPCR |
| Selección de arrestina beta basada en células | |
| (Kit de carga LiveBLAzer™-FRET B/G, solución D y otros materiales se requieren por separado; consulte el protocolo). Incluye: Células o Tango™ SSTR2-bla U2OS Protocolo de ensayo o Tango™ SSTR2-bla U2OS o Certificado de análisis Las células Tango™ SSTR2-bla U2OS se envían en hielo seco y deben almacenarse en nitrógeno líquido inmediatamente después de su recepción. |
|
| Fluorescente | |
| SSTR2 | |
| Origen del clon unicelular | |
| 2 x 106 células | |
| Temperatura ambiente | |
| Célula U2OS | |
| Tango™ |
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.