TNF alpha Monoclonal Antibody (XT22), Biotin, Invitrogen™

Descripción

MM350DB targets TNF Alpha in ELISA, Neutralization and WB applications and shows reactivity with mouse samples. The MM350DB immunogen is recombinant mouse TNF alpha. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified. MM350DB detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. The MM350DB mouse TNFa antibody (biotinylated conjugate of clone XT22) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM350C (clone XT3). Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Biotinylated antibody MM350DB (clone XT22) and antibody MM350C (clone XT3) have successfully been used in combination with recombinant mouse TNFa protein SMTNFA in ELISA applications.

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.