Learn More
Invitrogen™ Trappin-2/PI3 Human ELISA Kit
Sandwich ELISA Kit
Marca: Invitrogen™ EHPI3
Descripción
The Human Trappin-2/PI3 ELISA quantitates Hu PI3 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PI3. Principle of the method The Human PI3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Neutrófilos e inhibidores específicos de la elastasa pancreática de la piel. Puede prevenir la proteólisis tisular mediada por elastasa.Especificaciones
P19957 | |
5 pg/mL | |
ELISA sandwich de fase sólida | |
Kit de ELISA | |
Plasma, Sobrenadante, Suero | |
96 ensayos | |
ELISA, ensayos de proteínas, biología de proteínas, biología del cáncer, inmunología, neurociencia, neurología, detección de proteínas, análisis de proteínas | |
5266 | |
< 12 % | |
Placa de 96 pocillos recubiertos previamente, Estándar, Concentrado de diluyente de ensayo, Anticuerpo de detección biotinilado, SAV-HRP, Tampón de lavado, Cromógeno, Solución de parada, Cubiertas adhesivas para placas | |
96 pruebas | |
Otra investigación | |
De 2 °C a 8 °C | |
Humano | |
4 h 45 min |
6,86-5000 pg/ml | |
De 5 pg/mL a 5000 pg/ml | |
Biotina | |
Proteasas, peptidasas e inhibidores | |
Este kit de ELISA no muestra reactividad cruzada con las siguientes citoquinas probadas: angiogenina humana, BDNF, BLC, ENA-78, FGF- 4, IL-1 alfa, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, leptina (OB), MCP-1, MCP-3, MDC, MIP-1 alfa, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alfa, TNF-beta, TPO, VEGF. | |
Humano | |
Lector de microplacas colorimétrico | |
PI3 | |
< 10 % | |
Peroxidasa de rábano picante (HRP) | |
RUO | |
3,3 μl de plasma; 3,3 μl de suero; 100 μl de sobrenadante | |
ESI, SKALP, WAP3, WFDC14, cementoína | |
1 h 20 min |