Invitrogen™ XIAP Human ELISA Kit

Descripción

The Human XIAP ELISA quantitates Hu XIAP in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu XIAP. Principle of the method The Human XIAP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La proteína codificada por este gen es un miembro de una familia de proteínas que inhiben la apoptosis mediante la unión a factores asociados con el receptor del factor de necrosis tumoral TRAF1 y TRAF2. Esta proteína inhibe la apoptosis inducida por la menadiona, un potente inductor de radicales libres, e ICE. También inhibe al menos dos miembros de la familia caspasa de las proteasas de muerte celular, caspasa-3 y caspasa-7.