FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), NovaFluor™ Yellow 610, eBioscience™, Invitrogen™

Descripción

The AER-37 mAb reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. Fc epsilon RI apha is expressed on mast and basophil cells and is upregulated by the presence of IgE. Fc epsilon RI alpha forms a tetrameric complex with one beta and two gamma subunits. The beta and gamma subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The Fc epsilon RI complex plays an important role in triggering IgE-mediated allergic reactions. This AER-37 (CRA1) antibody has been reported for use in flow cytometric analysis. This AER-37 (CRA1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood cells. This can be used at 5 uL (0.2 ug) per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Add 5 uL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 uL of CellBlox Blocking Buffer per 100uL of cell sample containing 10^3 to 10^8 cells. Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow) Laser