missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Pierce™ Coomassie Brilliant Blue Dyes

Thermo Scientific Pierce Coomassie Brilliant Blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains.

178.00€ - 182.00€

Especificaciones

Contenido y almacenamiento Almacenar a temperatura ambiente.
Ubicación de detección Detección en gel, detección en solución, detección en transferencia
Método de detección Colorimétrico
Etiqueta o tinte Coomassie
Tipo de producto Tinción para geles de proteínas
Ver más especificaciones

Productos 2
Código de producto Marca Descripción Precio Cantidad y disponibilidad  
Código de producto Marca Descripción Precio Cantidad y disponibilidad  
11876744
Ver documentos Detalles de la promoción
Thermo Scientific™
20278
Colorante Coomassie Brilliant Blue R-250 Pierce
182.00€
50 g
En stock
para ver el stock
Añadir a la cesta
 
11886744
Ver documentos Detalles de la promoción
Thermo Scientific™
20279
Colorante azul brillante G-250 de Coomassie Pierce
178.00€
50 g
En stock
para ver el stock
Añadir a la cesta
 
Descripción

Descripción

Thermo Scientific Pierce Coomassie Brilliant Blue dyes are composed of one of the most common forms of coomassie dye, which is a key component of various colorimetric protein gel stains. Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine, and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features include:
Easy detection—develops intensely colored complexes with proteins
High sensitivity—can determine as little as 0.5 μg/cm2 of protein present in a gel matrix
Reversible staining—anion of Coomassie Brilliant Blue dye formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under proper conditions
Differentiation between bound and unbound dye—when dissolved in 0.01 M citrate buffer at pH 3.0, has an absorption maximum at 555 nm; protein-dye complex is characterized by a peak slightly broader than that of free dye with a maximum at 549 nm

Especificaciones

Especificaciones

Almacenar a temperatura ambiente.
Colorimétrico
Tinción para geles de proteínas
proteína
Detección en gel, detección en solución, detección en transferencia
Coomassie
50 g
Videos
SDS
Documentos

Documentos

Product Certifications

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.