Invitrogen™ Triiodothyronine (T3) Competitive ELISA Kit

Descripción

The Triiodothyronine (T3) ELISA quantitates T3 in serum,plasma,urine,dried fecal extracts,or cell culture medium.
Principle of the method
The Triiodothyronine (T3) Competitive ELISA research-use-only kit is designed to quantitatively measure T3 present in extracted serum and plasma,urine,extracted dried fecal samples,and tissue culture media samples. This kit measures total T3 in extracted serum and plasma and in extracted fecal samples. A T3 stock solution is provided to generate standard curves for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A T3-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a sheep antibody to T3 to each well. After a two-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound T3-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

Las hormonas tiroideas regulan una serie de actividades neuronales, metabólicas y de desarrollo en todo el cuerpo.La glándula tiroides sintetiza 2 hormonas:la tiroxina, que contiene 4 átomos de yodo (T4) y la triyodotironina (T3), que tiene 3 átomos de yodo.La producción de T3 en la glándula tiroides constituye aproximadamente el 20 % del total de T3; el resto se genera por la conversión (deyodación) de T4 a T3 en tejidos periféricos.Las hormonas tiroideas circulan principalmente unidas a proteínas portadoras (por ejemplo, globulina fijadora de tiroidina [TBG], prealbúmina y albúmina); mientras que solo una pequeña fracción circula sin consolidar.La forma libre de T3 es la fracción biológicamente activa.Aunque T3 y T4 están unidas a TBG, T3 está unida con menos firmeza que T4.La T3 total consta de fracciones enlazadas y no enlazadas.La triyodotironina (T3) es idéntica en todas las especies.