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Invitrogen™ CD14 Human ELISA Kit
Sandwich ELISA Kit
672.00€ - 4815.00€
Especificaciones
Número de acceso | P08571 |
---|---|
Sensibilidad del ensayo | 6 pg |
ensayo | ELISA sandwich de fase sólida |
Conjugado | Biotina |
Tipo de producto | Kit de ELISA |
Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
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Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
15404943
|
Invitrogen™
EHCD14 |
96 pruebas |
672.00€
1 set |
En stock
Inicie sesión para ver el stock |
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17145389
|
Invitrogen™
EHCD14X5 |
5 × 96 pruebas |
2635.00€
cada uno |
Fecha estimada de envío: 28-08-2024
Inicie sesión para ver el stock |
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18567914
|
Invitrogen™
EHCD14X10 |
10 x 96 Tests |
4815.00€
paquete de 10 |
Fecha estimada de envío: 29-08-2024 Inicie sesión para ver el stock |
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Descripción
The Human CD14 ELISA quantitates Hu CD14 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu CD14. Principle of the method The Human CD14 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.Especificaciones
P08571 | |
ELISA sandwich de fase sólida | |
Kit de ELISA | |
Este kit de ELISA no muestra reactividad cruzada con las siguientes citoquinas probadas: angiogenina humana, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alfa, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, leptina (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alfa, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alfa, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alfa, TNF-beta, TPO, VEGF. | |
ELISA, ensayos de proteínas, biología de proteínas, biología del cáncer, inmunología, neurociencia, neurología, detección de proteínas, análisis de proteínas | |
929 | |
< 12 % | |
Peroxidasa de rábano picante (HRP) | |
Apoptosis | |
Humano | |
4 h 45 min |
6 pg | |
Biotina | |
Proteína CD | |
Humano | |
Lector de microplacas colorimétrico | |
CD14 | |
< 10 % | |
RUO | |
De 2 °C a 8 °C | |
1 h 20 min |