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Gibco™ Human EGF Recombinant Protein
Recombinant Protein
Marca: Gibco™ PHG0311
269.80 EUR hasta el 2025-04-30
Use el código promocional "24155" para aplicar la oferta.
Descripción
Carrier-Free Reconstitution: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, PBS buffer to a concentration of 0.1-1.0 mg/mL. Make any further dilutions of the reconstituted protein in low endotoxin medium, or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA Storage: Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
Especificaciones
P01133 | |
protein with no preservative | |
6.2 kDa | |
Sequential Chromatography | |
<0.1 ng/ μg | |
EGF | |
Unconjugated | |
Recombinant | |
Carrier-Free | |
Wet Ice | |
Growth Factors & Receptors | |
Lyophilized | |
Stem Cell Research, Oncology | |
>95% by SDS-PAGE |
Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot | |
1950 | |
Human EGF | |
100 μg | |
AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone | |
ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells. | |
Human | |
4°C | |
<0.1 ng/μg | |
E. coli | |
Recombinant, Ligand | |
EGF (Epidermal Growth Factor) | |
Gibco™ |
Certificados
Se requiere un número de lote para mostrar resultados para certificados. Para encontrar su número de lote en pedidos anteriores, utilice el área de estado de pedidos.
Número de lote | Tipo de certificado | Fecha | Catalog Number |
---|---|---|---|
2970789 | Certificado de análisis | 31/01/2025 | PHG0311 |
2475286 | Certificado de análisis | 29/01/2025 | PHG0311 |
2489603 | Certificado de análisis | 29/01/2025 | PHG0311 |
2524683 | Certificado de análisis | 29/01/2025 | PHG0311 |
2567201 | Certificado de análisis | 29/01/2025 | PHG0311 |
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