MCP-4 Monoclonal Antibody, PeproTech®, Invitrogen™

Descripción

AA Sequence of recombinant protein: QPDALNVPST CCFTFSSKKI SLQRLKSYVI TTSRCPQKAV IFRTKLGKEI CADPKEKWVQ NYMKHLGRKA HTLKT. Preparation: Produced in mice immunized with highly pure Recombinant Human MCP-4 (CCL13). Anti-Human MCP-4 (CCL13)-specific antibody was purified from cell culture by Protein G affinity chromatography. Sandwich ELISA: Assuming 100 μL/well, a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-4 (CCL13) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MCP-4 (CCL13) (500-P04GBT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL. Western Blot: To detect Human MCP-4 (CCL13) by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 (CCL13) is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.

Monocyte Chemotactic Protein 4 (MCP-4), also called CCL13, is induced by inflammatory proteins such as IL-1 and TNFalpha. MCP-4 is a ligand for three different G protein coupled receptors, CCR2, CCR3 and CCR5. MCP-4 activates signaling in monocytes, T lymphocytes, eosinophils and basophils and this signaling is associated with the allergic response.