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R&D Systems™ Recombinant Human Thrombopoietin His (HEK-293) Protein
Analyzed by SEC-MALS. His-tag (HEK-293-expressed)
Marca: R&D Systems™ 288-TPH-050
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Descripción
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 0.0500-0.500 ng/mL.
Especificaciones
| P40225.1 | |
| Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | |
| MolecularWeight-observed: 26-30 kDa, under reducing conditions., MolecularWeight-theroretical: 19 kDa | |
| Human embryonic kidney cell, HEK293-derived human Thrombopoietin/Tpo protein Ser22-Leu195, with a C-terminal 6-His tag | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Unconjugated | |
| >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
| Bioactivity | |
| 7066 | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, megakaryocyte stimulating factor, MGDF, MGDFC-mpl ligand, MKCSF, MK-CSF, ML, MPL ligand, MPLLG, MPLLGMGC163194, Myeloproliferative leukemia virus oncogene ligand, THCYT1, THPO, thrombopoietin, thrombopoietin nirs variant 1, Tpo, TPOMKCSF | |
| Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes (at 500 μg/mL in PBS.) | |
| Thrombopoietin/THPO |
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Título del producto
R&D Systems™ Recombinant Human Thrombopoietin His (HEK-293) Protein > 50 μg
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