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IBA Lifesciences Vectores StarGate pYSG-IBA para expresión en levadura
Diseñado para la expresión de alto nivel de proteínas recombinantes en levaduras mediante la introducción del gen de interés bajo el control del promotor CU++-inducible CUP1.
220.00€ - 266.00€
Especificaciones
| Formato | Líquido |
|---|---|
| Para utilizar con (equipo) | Sistema StarGate™, células huéspedes de levaduras |
| Agente inductor | Sulfato de cobre |
| Gen marcador | leu2-d y URA3 |
| pH | 8 |
| Código de producto | Marca | Vector | Precio | Cantidad y disponibilidad | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Código de producto | Marca | Vector | Precio | Cantidad y disponibilidad | ||||||
|
15613808
|
IBA Lifesciences
5-4600-001 |
pYSG-IBA wt1 | N/A | N/A | N/A | |||||
|
15623808
|
IBA Lifesciences
5-4603-001 |
pYSG-IBA 3 | N/A | N/A | N/A | |||||
|
15633808
|
IBA Lifesciences
5-4605-001 |
pYSG-IBA 5 | N/A | N/A | N/A | |||||
|
15643808
|
IBA Lifesciences
5-4623-001 |
pYSG-IBA 23 | N/A | N/A | N/A | |||||
|
15653808
|
IBA Lifesciences
5-4625-001 |
pYSG-IBA 25 | N/A | N/A | N/A | |||||
|
15663808
|
IBA Lifesciences
5-4633-001 |
pYSG-IBA 33 | N/A | N/A | N/A | |||||
|
15673808
|
IBA Lifesciences
5-4643-001 |
pYSG-IBA 43 | N/A | N/A | N/A | |||||
|
15683808
|
IBA Lifesciences
5-4645-001 |
pYSG-IBA 45 | N/A | N/A | N/A | |||||
|
15693808
|
IBA Lifesciences
5-4662-001 |
pYSG-IBA 62 | N/A | N/A | N/A | |||||
|
15603818
|
IBA Lifesciences
5-4663-001 |
pYSG-IBA 63 | N/A | N/A | N/A | |||||
|
15613818
|
IBA Lifesciences
5-4664-001 |
pYSG-IBA 64 | N/A | N/A | N/A | |||||
|
15623818
|
IBA Lifesciences
5-4665-001 |
pYSG-IBA 65 | N/A | N/A | N/A | |||||
|
15633818
|
IBA Lifesciences
5-4703-001 |
pYSG-IBA 103 | N/A | N/A | N/A | |||||
|
15643818
|
IBA Lifesciences
5-4705-001 |
pYSG-IBA 105 | N/A | N/A | N/A | |||||
|
15653818
|
IBA Lifesciences
5-4723-001 |
pYSG-IBA 123 | N/A | N/A | N/A | |||||
|
15663818
|
IBA Lifesciences
5-4743-001 |
pYSG-IBA 143 | N/A | N/A | N/A | |||||
|
15673818
|
IBA Lifesciences
5-4745-001 |
pYSG-IBA 145 | N/A | N/A | N/A | |||||
|
15683818
|
IBA Lifesciences
5-4762-001 |
pYSG-IBA 162 | N/A | N/A | N/A | |||||
|
15693818
|
IBA Lifesciences
5-4764-001 |
pYSG-IBA 164 | N/A | N/A | N/A | |||||
|
15603828
|
IBA Lifesciences
5-4767-001 |
pYSG-IBA 167 | N/A | N/A | N/A | |||||
|
15613828
|
IBA Lifesciences
5-4768-001 |
pYSG-IBA 168 | N/A | N/A | N/A | |||||
Descripción
pYSG-IBA yeast expression vectorsThe pYSG-IBA vector allows the high-level expression of proteins in yeast.
- The vector carries the copper inducible promoter (CUP1) for controlled high-level expression.
- The expressed recombinant protein will be localized in the cytoplasm.
- High-level expression in yeast with high copy number
- Favors correct protein folding and the production of soluble proteins - inclusion bodies rarely form
- Tightly regulated expression due to Cu++-inducible CUP1 promoter
- Ampicillin resistance gene and markers Leu2-d and URA3 in every vector
- Leu2-d auxotrophy marker for selection to increase plasmid copy number during expression
- URA3 auxotrophy marker for selection after transformation
Please note that cloning into pYSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pYSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.
Especificaciones
| Líquido | |
| Sulfato de cobre | |
| 8 | |
| Vector Acceptor para la expresión en levaduras | |
| de 2 °C a 8 °C para uso frecuente, –20 °C para almacenamiento a largo plazo | |
| 5 μg | |
| Clonación de genes de interés para su uso en la expresión funcional, purificación, detección, inmovilización o separación de proteínas recombinantes |
| Sistema StarGate™, células huéspedes de levaduras | |
| leu2-d y URA3 | |
| CUP1 | |
| Tampón TE de 20 μl: 10 mm de Tris-HCl, 1 mm de EDTA/5 μg | |
| 250 ng/μl | |
| Transparente |
Seguridad y manipulación
missing translation for 'shelfLife' : 12 meses
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Título del producto
IBA Lifesciences Vectores StarGate pYSG-IBA para expresión en levadura