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Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)
Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.
Marca: Thermo Scientific™ EP0072
146.25 EUR hasta el 2024-12-20
Use el código promocional "21651" para aplicar la oferta.
Description
Thermo Scientific Taq DNA Polymerase, native, is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5' to 3' synthesis of DNA, has no detectable 3' to 5' exonuclease (proofreading) activity, and possesses low 5' to 3' exonuclease activity. In addition, the polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA Polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E. coli.
Características destacadas
- Termoestable: la vida media es superior a 40 minutos a 95 °C
- Genera productos de PCR con salientes de 3'-dA.
- Se suministra con dos tampones: tampón de Taq 10X con KCl y tampón de Taq 10X con (NH4)2SO4. Este último permite llevar a cabo la PCR con una amplia gama de concentraciones de magnesio y disminuye el cebado inespecífico.
- Incorpora nucleótidos modificados (por ejemplo, nucleótidos marcados con biotina, digoxigenina y fluorescencia)
Specifications
Contiene: • 100 μl de ADN polimerasa Taq, nativa (5 U/μL) • 2 x 1,25 ml de tampón Taq 10X con KCl • 2 x 1,25 ml de tampón Taq 10X con (NH4)2SO4 • 2 x 1,25 ml de 25 mm de MgCl2 • 0,25 ml de 20 mg/ml de BSA Almacenar a - 20 °C. |
|
ADN polimerasa Taq | |
ADN polimerasa | |
PCR | |
Standard PCR |
With BSA | |
de 72 °C | |
300 U | |
Dry Ice | |
5 U/µl |
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For Research Use Only. Not for use in diagnostic procedures.