GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA (Division Arrested) cells and FPRL-1-Gα15-NFAT-bla CHO-K1 cells contain the human Formyl Peptide-like 1 receptor (FPRL-1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.
Marca: Thermo Scientific™ K1357
This cell line is available as dividing cells by requesting a quote for K1189 from dividingcells@invitrogen.com
GeneBLAzer™ FPRL-1-Gα15 CHO-K1 DA (Division Arrested) cells and FPRL-1-Gα15-NFAT-bla CHO-K1 cells contain the human Formyl Peptide-like 1 receptor (FPRL-1) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cells (Cat. No. K1213) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element, in addition to the promiscuous G-protein, Gα15. Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.
DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both FPRL-1-Gα15 CHO-K1 DA cells and FPRL-1-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of WKYMV(M); (Figure 1). In addition, FPRL-1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
The FPRL-1 receptor has been implicated in a variety of inflammation and neurological disorders, including Alzheimer's disease.
| NM_001462 | |
| Células de división detenida | |
| Kit de ensayo GeneBLAzer™ FPRL-1-Gα±15 CHO-K1 DA, 1 placa, cantidad de células ∽ 2 400 000 células/vial, línea de células CHO-K1, método de detección fluorescente, objetivo quimiomodulable GPCR, indicador BLA (beta-lactamasa), almacenamiento en nitrógeno líquido | |
| Análisis celular, adquisición de imágenes celulares, ensayos celulares con indicador, ensayos celulares y de lactamasa, descubrimiento y desarrollo de fármacos, identificación y validación de objetivo y potencial, biología de receptores acoplados a proteínas G, ensayos celulares con indicadores GPCR | |
| FPRL-1 | |
| Origen del clon unicelular | |
| 1 placa | |
| CHO-K1 | |
| Kit de ensayo GeneBLAzer™ FPRL-1-Gα±15 CHO-K1 DA | |
| GeneBLAzer™ |
| Gen informador de beta lactamasa a base de células | |
| Nitrógeno líquido | |
| Fluorescente | |
| 3269 | |
| Kit de ensayo GeneBLAzer™ FPRL-1-Gα±15 CHO-K1 DA | |
| GeneBLAzer™ | |
| BLA (betalactamasa) | |
| FPRL-1 | |
| CHO-K1 | |
| GPCR |
Catalog No. 12559069
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Se requiere un número de lote para mostrar resultados para certificados. Para encontrar su número de lote en pedidos anteriores, utilice el área de estado de pedidos.
| Número de lote | Tipo de certificado | Fecha | Catalog Number |
|---|---|---|---|
| 397252 | Certificado de análisis | 15/04/2017 | K1357 |
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
