PPAR delta Polyclonal Antibody, Invitrogen™

Description

PA1-823A detects peroxisome proliferator activated receptor (PPAR) delta from rat, human and mouse cells. This antibody does not detect PPAR alpha or PPAR gamma. PA1-823A has been successfully used in Western blot and immunohistochemical and immunofluoresence procedures. By Western blot, this antibody detects an ∽49 kDa protein representing PPAR delta from NIH-3T3 cell lysate. Immunolocalization yeilds predominant nuclear staining. The PA1-823A immunogen is a synthetic peptide corresponding to residues M(1) E Q P Q E E T P E A R E E(14) C of mouse PPAR delta. This sequence is 86% conserved in human PPAR delta. PA1-823A immunizing peptide (Cat. # PEP-026) is available for use in neutralization and control experiments.

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.